Quadrupole Magnetic Sorter

Quadrasep Magnetic cell Sorter

The Quadrasep Magnetic cell Sorter (QMS) purifies cells labeled with magnetic particles. The cells are sorted based on how fast the labeled cells move in a magnetic field, a property known as “Magnetophoretic Mobility.”

This allows for fast, gentle, ultra-high speed cell sorting based on immunological biomarkers. Three fractions are obtained in the sorting process: low mobility (negatively selected cells), medium mobility (positively selected cells), and high mobility (cells targeted for depletion). Depending on your application, any of the three fractions can be recovered for further use or analysis.

How Our Technology Works

1. Magnetic particles are conjugated to affinity biomarkers
2. The cell sample is prepared
3. The cells are labeled with the conjugated particles
4. The cells are analyzed using Hyperflux Magnetic Velocimetry to obtain a mobility distribution profile
5. Sorting gates are selected from the mobility distribution profile to determine Quadrasep settings
6. The cells are sorted based on how fast they are laterally deflected by the magnetic field while travelling through a laminar flow stream
7. The process results in three fractions
   • Fraction 1: low or non-expressing cells that are not deflected
   • Fraction 2: desired antigen expressing cells with moderate magnetic label
   • Fraction 3: trapped cells with highly magnetic label